Protein_Domain

Part:BBa_K3019003:Design

Designed by: Valeriia Leoshko   Group: iGEM19_Tartu_TUIT   (2019-10-13)


α-factor pre-pro signal 3HA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Illegal cutting site removal


Source

Alfa factor pre-pro signal sequence was taken from yeast genomic DNA and 3HA tag sequence was kindly provided by professor Mart Loog research group. 3HA tag was placed to pre-pro signal C-terminal. The whole sequence was ordered from IDT.

References